Influence of manufactured sand with deferent stone powder contents and MB values on properties of high-strength concrete is studied. The results show that the proper amount of stone powder can make up the roughness of the surface of manufactured sand, and improve the workability of fresh concrete. When the content of stone powder is higher, the workability of fresh concrete gradually deteriorates due to the adsorption of stone powder on additives and free water. With the increasing of stone powder content, the corresponding concrete compressive strength decreases, and the splitting tensile strength and elastic modulus increase first and then decrease. In this experiment, the optimum value of stone powder in manufactured sand is 3%~6%. With the increasing of MB value, the workability of fresh concrete deteriorates rapidly, and the compressive strength and splitting tension of concrete decrease. When the MB value is lower than 1.1, the elastic modulus of concrete has no obvious change with the increasing of MB value. When the MB value is higher than 1.1, the elastic modulus decreases rapidly. In this experiment, the optimum MB value of manufactured sand should be lower than 0.75.