微生物诱导碳酸钙沉积（MICP）技术因具有低能耗、低污染等环境友好性优势成为文物保护、水处理、混凝土修补加固等领域的研究热点。MICP的矿化效果受多因素的制约与影响。本文开展了环境温度、pH值等因素对细菌生长和微生物矿化的影响研究。结果表明，环境温度不超过40℃时对菌液脲酶活性影响不大，42.5~45℃时6h后脲酶活性急剧降低，超过45℃时30min后菌液浓度及脲酶活性均大幅下降，直至失活；pH值越大，脲酶活性衰减越明显，pH≥11时脲酶活性急剧下降，大量的细菌不能承受高碱性环境进一步分解死亡。MICP技术在30℃时碳酸钙产量最高，在pH值为11~13的环境中细菌很难生长繁殖，碳酸钙生成量较低。

Microbial-induced calcium carbonate precipitation (MICP) technology has become a focus of research in the fields of historic preservation, pollutant treatment and concrete repair & reinforcement, due to its low pollution, low energy consumption and environmental friendliness. However, the mineralization effect of MICP is restricted and affected by many factors. In this paper, the effects of temperature, pH value and other factors on bacterial growth and microbial mineralization are studied. The results show that the urease activity is not affected when the ambient temperature is not more than 40℃ and decreases sharply at 42.5~45℃ after 6h. When the temperature exceeds 45℃ for 30min, the concentration of bacterial liquid and urease activity decrease significantly. With the increase of pH value, the urease activity decrease obviously. When pH≥11, a large number of bacteria cannot withstand the high alkaline environment and gradually decompose and die. MICP technology has the highest calcium carbonate output at 30℃. In the environment with pH value of 11~13, it is difficult for bacteria to grow and reproduce, and the output of calcium carbonate is low.

TU528

流域水循环模拟与调控国家重点实验室自主研究课题（SKL2022TS06）；中国水科院基本科研业务费项目（SM0145B022021）